质量标准:【产品介绍】4E-BP1 (eIF4E-binding protein) also known
as PHAS, is a 10-12 kDa acidic protein that compete
with eIF4G for binding of eIF4E to the mRNA 5' cap
structure (1). Binding of the 4E-BPs to eIF4E is
reversible and is dependent on the phosphorylation
status of 4E-BP. Non-phosphorylated 4E-BP1 will
bind strongly to eIF4E, while the phosphorylated
form will not (2). Akt, TOR, MAP kinase, S6 kinase,
and Cdc2 are known kinases capable of inactivating
4E-BP1 binding to eIF4E by phosphorylating either
threonines 35, 45, 69 or serine 64. However, not
all phosphorylation events equally block the
4EBP1-eIF4E interaction (3-4)
Recommended Applications
WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors
WB: 1:500 -1000
IHC: 1:100-250
ICC: 1:100-250
IP: 1:50
FC: 1:20
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Specificity
A synthetic peptide corresponding to residues near
the N-term of human4E-BP1 was used as immunogen.
贮存:Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.