质量标准:【产品介绍】Akt1/PKBa is a Ser/Thr protein kinase,
whose kinase activity plays a key role in various
cellular functions, including apoptosis, glycogen
synthesis, a n d cell growth. Akt1/PKBa is activated
by various growth a n d survival fact o r s (1). Phosph
o r ylation on Thr308, Ser473 a n d Tyr474 is required
f o r full activity. Akt1/PKBa inhibits apoptosis by
phosph o r ylating a n d inactivating several
targets, including caspase-9 (2). Akt1/PKBa
regulates glycogen synthesis by phosph o r ylating
a n d inactivating glycogen synthase kinase-3a
a n d b (3, 4). Three mammalian Akt1/PKBa isof o r ms
have been identified.
WB: 1:5,000 – 10,000
A. Western blot analysis on NIH 3T3 cell lysates
using anti-Phospho-Akt1 (pT308) RabMAb
(cat. #2214-1), 1:10,000 dilution. Cells were
either (A) untreated (B) treated with PDGF