质量标准:【产品介绍】Akt1/PKBa is a Ser/Thr protein kinase
whose kinase activity plays a key role in various
cellular functions, including apoptosis, glycogen
synthesis, a n d cell growth . Akt1/PKBa is activated
by various growth a n d survival fact o r s (1).
Phosph o r ylation on Thr308, Ser473 a n d Tyr474
is required f o r full activity. Akt1/PKBa
inhibits apoptosis by phosph o r ylating a n d
inactivating several targets, including
caspase-9 (2). Akt1/PKBa regulates glycogen synthesis
by phosph o r ylating a n d inactivating glycogen
synthase kinase-3a a n d b (3,4). Three mammalian
Akt1/PKBa isof o r ms have been identified.
WB: 1:5000 - 10,000
IHC: 1:100 250
ICC: 1:100 250
A. Immunohistochemical analysis of untreated
cervical carcinoma using anti-Phospho-Akt1 (pS473)
RabMAb (cat. # 2118-1).
B. Immunohistochemical analysis of
Phosphatase- treated cervical carcinoma
using anti-Phospho-Akt1 (pS473) RabMAb (cat. # 2118-1).
C. Western blot analysis on 3T3 cell lysates
using anti-Phospho-Akt1 (pS473) RabMAb
(cat. #2118-1), 1:10,000 dilution. Cells were
either (A) untreated o r (B) treated with PDGF.