质量标准:【产品介绍】Microtubules are essential parts in
eukaryotic cell structures, transp o r tation, a n d
mitosis a n d consists mainly of 2 soluble protein
subunits, alpha a n d beta tubulin. Beta-tubulin binds
to alpha tubulin to f o r m tubulin heterodimer which
is post-translationally modified (1). The tubulin
dimer complex binds to GTP a n d assembles onto the
positive ends of microtubules. After inc o r p o r
ation into the microtubules, bound GTP is hydrolyzed by
beta tubulin. The stability of the dimer in the
microtubules is depended on presence of beta tubulin,
where dimer with GTP bound beta-tubulin is stable
to microtubule inc o r p o r ation (2). Class III
beta tubulin (beta III-tubulin) is a vertebrate
tubulin Isotype specific to the neurons a n d
mammalian testis cells, making it an ideal neuronal
marker. Overexpression of class III beta tubulin
is associated with the resistances of
microtubule-targeted cancer drugs in lung cancer cell
lines, breast cancer cell lines, a n d ovarian tum o r s (3).
WB: 1:20,000
IHC: 1:50 100
ICC: 1:100 250
Flow Cytometry: 1:10
A. Western blot analysis on HeLa cell lysate using
anti- β-Tubulin, class III RabPAb (cat. # 1862-1),
dilution 1:1,000.
B. Immunohistochemical analysis of paraffin-embedded
human breast carcinoma using anti-Tubulin beta, class
III RabPAb (cat# 1862-1)
C. Imunoflu o r escent staining of HeLa cells using
anti- β-Tubulin, class III RabPAb (cat# 1862-1)