质量标准:【产品介绍】DEAD box proteins, characterized by
the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative
RNA helicases. They are implicated in a number of
cellular processes involving alteration of RNA
secondary structure such as translation initiation,
nuclear and mitochondrial splicing, and ribosome
and spliceosome assembly. Based on their
distribution patterns, some members of this DEAD box
protein family are believed to be involved in
embryogenesis, spermatogenesis, and cellular growth
and division. DDX50 is a DEAD box enzyme that may
be involved in ribosomal RNA synthesis or processing.
DDX50 and DDX21, also called RH-II/GuA, have
similar structures and are in tandem orientation (1).
Recommended Applications
WB, ICC
Applications and Recommended Dilution Factors
WB: 1:50,000 - 200,000
ICC: 1:250 - 500
Species Reactivity *
Human
*Cross reactivity determined by western blot only.
Products Data
Western blot analysis on (A) HeLa, (B) 293T, (C) K562,
and (D) Jurkat cell lysates using anti-DDX50 RabMAb
(cat. #3903-1).
Specificity
A synthetic peptide corresponding to residues in human
DDX50 was used as an immunogen.
贮存:Storage Condition and Buffer
Store at -20° C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.