质量标准：【产品介绍】DLC8 protein bound efficiently to gephyrin in
in vitro binding assays a n d colocalized with
gephyrin during coexpression in HEK293 cells. The
binding site f o r DLC8 was mapped to a fragment of 63
amino acids within the central linker domain of gephyrin.
In hippocampal neurons, endogenous DLC8 protein was
enriched at synaptic sites identified by synaptophysin
a n d gephyrin immunostaining. Because DLC8 has
been described as stoichiometric components of
cytoplasmic dynein a n d myosin-Va complexes,
results suggest that mot o r proteins are involved in
the subcellular localization of gephyrin (1). DLC8
was identified as a transp o r t molecule in the
cytoplasm. DLC8 co-localizes with TRPS1 in
dot-like structures in the cell nucleus. In an electroph
o r etic mobility shift assay it was shown that
the interaction of DLC8 a n d TRPS1 lowers the binding
of TRPS1 to the GATA consensus sequence. In addition DLC8
is able to suppress the transcriptional repression
activity of TRPS1 (2).
WB: 1:10,000
IHC: 1:100 250
ICC: 1:100 250
IP: 1:100
A. Western blot analysis on HeLa cell lysate using
anti-DLC8 RabMAb (cat. # 2003-1), dilution 1:10,000.
B. Immunohistochemical staining of paraffin-embedded
human liver using anti-DLC8 RabMAb (cat. #2003-1).
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