质量标准:【产品介绍】Amyloid beta A4 precursor protein-binding
family B member 1 (FE65) is a member of a family
of multidomain adaptor proteins (FE65, FE65L1, and
FE65L2) that form multiprotein complexes with a range
of functions. FE65 is highly expressed in the brain and
it has three protein-protein interaction domains:
a WW domain and two PID/PTB domains. Through the
PTB2 domain, FE65 interacts with the Alzheimer’s
disease amyloid precursor protein (APP) intracellular
domain (AICD) and can alter APP processing. The WW domain
is responsible for the interaction with various
proteins, which in turn interacts with the cytoskeleton.
The second PID/PTB domain of FE65 binds to the
transcription factor, CP2/LSF/LBP1 protein (1, 2). FE65
also plays a central role in the response to DNA damage
by translocating to the nucleus and inducing apoptosis (3).
Recommended Applications
WB, IHC, ICC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:50 - 100
ICC: 1:50
Species Reactivity
Human
Products Data
Western blot analysis on SH-SY5Ycell lysates using
anti-FE65 RabMAb (cat. #2660-1).
Immunohistochemical analysis of paraffin-embedded
human brain tissue using anti-FE65 RabMAb (cat. #2660-1).
Specificity
A synthetic peptide corresponding to residues in human
FE65 was used as an immunogen.
贮存:Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.