质量标准：【产品介绍】GRP170 / ORP150 belongs to the heat
shock protein 70 family. It uses alternative
transcription start sites. A cis-acting segment found in
the 5' UTR is involved in stress-dependent
induction, resulting in the accumulation of this protein
in the endoplasmic reticulum (ER) under hypoxic
conditions. GRP170 / ORP150 is thought to play an
important role in protein folding and secretion in the
ER. Since suppression of the protein is associated
with accelerated apoptosis, it is also suggested to have
an important cytoprotective role in hypoxia-induced
cellular perturbation. This protein has been shown to
be up-regulated in tumors, especially in breast tumors,
and thus it is associated with tumor invasiveness.
GRP170 / ORP150 also has an alternative
translation initiation site, resulting in a protein
that lacks the N-terminal signal peptide. This
signal peptide-lacking protein, which is only 3 amino
acids shorter than the mature protein in the ER, is
thought to have a housekeeping function in the cytosol.
In rat, this protein localizes to both the ER by a
carboxy-terminal peptide sequence and mitochondria by
an amino-terminal targeting signal (1).
Recommended Applications
WB, IHC, FC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
IHC: 1:250 - 500
FC: 1:10 - 100
Species Reactivity *
Human, Mouse, Rat
*Species cross-reactivity is based on WB analysis
Products Data
Western blot analysis on (A) fetal liver, (B) rat liver,
(C) rat pancreas, (D) 293T, (E) MCF-7, (F) MDA-MB-435S,
(G) SH-SY5Y, and (H) HeLa lysates using anti-GRP170 /
ORP150 RabMAb (cat. #3905-1).
Immunohistochemical analysis of paraffin-embedded
human liver tissue using anti-GRP170 / ORP150 RabMAb
(cat. #3905-1).
Specificity
A synthetic peptide corresponding to residues in
human GRP170 / ORP150 was used as an immunogen.
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