IRS-2 (IRS2)

分子式：

分子量：

别名：
IRS-2

产品订购：

品名	规格	包装	单价	货期
IRS-2 (IRS2)		100ul	询价	现货

性状：
【产品类型】Rabbit Monoclonal IgG
【反应特异性】Human
【克隆 ID】EP903Y
【用途】WB , ICC , IP
【产品性状】
【阳性对照】
【产品浓度】
【细胞定位】
【抗原分子量】170 185 kDa

质量标准：
【产品介绍】Insulin recept o r substrates (IRS), the
maj o r intracellular substrates of the insulin recept
o r (IR), are adapt o r proteins that transduce signals
from the IR to downstream effect o r s that are imp o r
tant f o r the biological effect of insulin (1-2).
After insulin stimulation, IRS proteins are rapidly phosph
o r ylated on multiple tyrosine residues. Once phosph
o r ylated, IRS proteins bind a n d activate Grb-2, SHP2
a n d the PI3-K p85 subunit (2-3). Sequences of IRS-2
a n d IRS-1 reveal a highly conserved amino
terminus containing a pleckstrin-homology domain
a n d a phosphotyrosine-binding domain, a n d a
po o r ly conserved carboxy terminus containing
several tyrosine phosph o r ylation motifs. IRS-2
is expressed in many cells, including tissues from
IRS-1-/- mice, a n d may be essential f o r signaling
by several recept o r systems such as insulin a n d
cytokine (1).

Recommended Applications
WB, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:5,000-10,000
ICC: 1:250 - 500
IP: 1:50
Species Reactivity *
Human
**Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on 293 + insulin cell lysate
using anti-IRS-2 RabMAb (cat. # 1802-1), dilution 1:100,000.

Specificity
A synthetic peptide corresponding to residues near
the N-terminus of human IRS-2 was used as an immunogen.

贮存：
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.