质量标准:【产品介绍】C-Jun N-terminal kinases (JNK) are
distantly related to mitogen-activated protein
kinases (ERKs) and are activated by dual phosphorylation
on Tyr and Thr. The JNK protein kinase group consists
of three isoforms (JNK1, 2, and 3) and
regulates transcription activity of the AP-1 complex,
and release and regulation of cytochome c (1). JNK
becomes activated in vivo in response to
pro-inflammatory cytokines or cellular stresses. Its
full activation requires the phosphorylation of a
threonine and a tyrosine residue in a Thr-Pro-Tyr
motif, which can be catalysed by the protein kinases
mitogen-activated protein kinase kinase MKK4 and MKK7
(2). Unlike other JNK isoforms, JNK3 is only expressed
in the heart and nervous system. JNK3 has been implicated
in stress-induced neuronal apoptosis (3).
Recommended Applications
WB, IP
Applications and Recommended Dilution Factors
WB: 1:1,000
IP: 1:20
Species Reactivity *
Human, Mouse, Rat
*Cross reactivity determined by western blot only.
Products Data
Western blot analysis on NIH/3T3 cell lysates
using anti-Phospho-JNK1 (pY185) / JNK2 (pY185) /JNK3
(pY223) RabMAb (cat. #2155-1). Cells were either
(A) untreated (B) treated with Anisomycin.
Specificity
A synthetic phospho-peptide corresponding to
residues surrounding tyrosine 223 of human JNK3 was used
as immunogen. The antibody will detect JNK1 (pY185), JNK2(pY185) and JNK3 (pY223).