Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
ACADM antibody, ACAD1 antibody, FLJ18227 antibody, FLJ93013 antibody, FLJ99884 antibody, MCAD antibody, MCADH antibody, Medium-chain specific acyl-CoA dehydrogenase, mitochondrial antibody, antibody

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human , Mouse , Rat
【克隆 ID】EPR3708
【用途】WB , IHC , ICC , IP
【抗原分子量】43kDa

Quality Standard：
【产品介绍】Medium-chain specific acyl-CoA
dehydrogenase (ACADM) is a homotetramer enzyme of
the mitochondrial flavoenzyme family that catalyzes
the initial reaction in medium chain fatty acid beta-oxidation (1, 2). ACADM is specific for acyl
chain lengths of 4 to 16 (3). It utilizes the
electron transfer flavoprotein (ETF) as an electron
acceptor that transfers the electrons to the
main mitochondrial respiratory chain via
ETF-ubiquinone oxidoreductase (3). Defects in the ACADM
gene cause medium-chain acyl-CoA dehydrogenase
deficiency (MCADD), a metabolic disorder characterized
by fasting hypoglycemia (3). Mutation analysis can
diagnose MCADD, which is currently screened in
newborns, since its monogene may be modified by
genetic variations (4).

Recommended Applications

WB, IHC, ICC, IP
Applications and Recommended Dilution Factors

WB: 1:10,000-50,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:10 - 100
Species Reactivity *

Human, Mouse, Rat

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data
Western blot analysis on (A) human heart, (B) fetal
liver, (C) HeLa, (D) HepG2, and (E) K562 cell lysates
using anti-ACADM RabMAb (cat. # 2833-1).
Immunohistochemical analysis of paraffin-embedded
human liver tissue using anti-ACADM RabMAb (cat. #2833-1).
Immunofluorescent staining of HeLa cells using
anti-ACADM RabMAb (cat. #2833-1).

Specificity

A synthetic peptide corresponding to residues in human
ACADM was used as an immunogen.

Storage：
Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05%
BSA. Stable for 12 months from date of receipt.