Quality Standard：【产品介绍】Microtubules are essential parts in
eukaryotic cell structures, transp o r tation,
a n d mitosis. It consists mainly of 2 soluble
protein subunits, alpha a n d beta tubulin. Beta
tubulin binds to alpha tubulin to f o r m
tubulin heterodimer which is post-translationally
modified (1). The tubulin dimer complex binds to GTP
a n d assembles onto the positive ends of
microtubules. After inc o r p o r ation into
the microtubules, bound GTP is hydrolyzed by beta
tubulin. The stability of the dimer in the microtubules
is depended on presence of beta tubulin, where dimer
with GTP bound beta-tubulin is stable to microtubule inc
o r p o r ation (2). F o r both type of tubulin, the
carboxy-terminal tail is pos-translationally modified
to regulate associated protein binding at
microtubule surface (3). A mutation on alpha tubulin
has been linked to abn o r mal neuronal migration
a n d neurodevelopmental dis o r ders in human (4).
WB: 1:50,000
IHC: 1:250 500
ICC: 1:250 500
Flow Cytometry: 1:20
IP: 1:50
A. Western blot analysis on HeLa cell lysate using
anti- α-Tubulin RabMAb (cat. # 1878-1),
dilution 1:50,000.
B. Immunohistochemical analysis of paraffin-embedded
human gastric carcinoma using anti- α-Tubulin
RabMAb (cat# 1878-1).
A. Imunoflu o r escent staining of HeLa cells using
anti- α-Tubulin RabMAb (cat# 1878-1)
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