Quality Standard:【产品介绍】Apaf-1, o r apoptotic protease activating fact
o r 1, is a human protein homolog of the C.
elagans apoptotic regulat o r y protein CED-4 that
activates caspase-9 during apoptosis (1). Cytosolic Apaf-1
f o r ms an oligomeric complex with caspase-9 in
the presence of cytochrome c a n d dATP, which leads
to caspase-9 activation a n d subsequent activation
of caspase-3 (2, 3). Oligomeric Apaf-1 a n d
pro-caspase-9 bind to each other via their
respective NH2-terminal CARD domains, consequently
enhancing the catalytic activity of caspase-9 (4),
leading to activation of the caspase signaling cascade
a n d apoptosis.
WB: 1:500
IHC: 1:50-1:100
ICC: 1:50-1:100
A. Western blot analysis on HeLa cell lysate
using anti-Apaf-1 RabMAb (cat. #1038-1); dilution 1:500.
B. Immunohistochemical analysis of paraffin-embedded
stomach adenocarcinoma using anti-Apaf-1 RabMAb
(cat. #1038-1).