Quality Standard：【产品介绍】Btk (Bruton tyrosine kinase also known as BPK
o r ATK) is a member of the TEC family of non-recept
o r protein tyrosine kinase which includes Bmx, Itk
a n d TEC. Btk is composed of a pleckstrin domain, an SH3
a n d SH2 domain, a catalytic kinase domain a n d a
TEC homology domain (1). BTK plays a crucial role in B
cell activation a n d development (2). Similar to
Etk-TNFR2 interaction, Btk interacts with Fas, a member
of the TNFR family involved in apoptosis in a lig
a n d -independent manner (3) Btk can be activated
through intramolecular autophosph o r ylation at
tyrosine 551 a n d that Btk autophosph o r ylation
is required f o r Btk catalytic functions (4). Mutations
in the Btk gene have been linked to severe
developmental blocks in human B-cell ontogeny leading
to X-linked agammaglobulinemia (XLA). Patients with
this disease are unusually prone to bacterial infection
but not to viral infection (5).
WB: 1:2,000
ICC: 1:100-250
A. Western blot analysis of anti-Btk phospho (pY551)
RabMAb (cat. #1685-1), 1:2,000 dilution A: K562 cell
lysate non-treated B: K562 cell lysate + Pervanadate
C: Jurkat cell lysate non-treated D: Jurkat cell
lysate + Pervanadate
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