Quality Standard：【产品介绍】Caldesmon is a smooth muscle and
nonmuscle regulatory protein that interacts with
actin, myosin, tropomyosin, and calmodulin (1,2).
Smooth muscle caldesmon is an elongated molecule with
a calmodulin, tropomyosin, and actin-binding region at
the C-terminus and a myosin-binding domain at the
N-terminus (3). Caldesmon stimulates actin binding
of tropomyosin which increases the stabilization of
actin filament structure. In muscle tissues,
caldesmon inhibits the actomyosin ATPase by binding
to F-actin. This inhibition is reduced by
calcium-calmodulin and is promoted by tropomyosin
(4). Phosphorylation of non-muscle caldesmon has been
linked to reduction of caldesmon's binding properties
to actin, myosin, and calmodulin. Also,
caldesmon's inhibition of actomyosin ATPase is reduced
with its Phosphorylation (5).
Recommended Applications
WB, ICC
Applications and Recommended Dilution Factors
WB: 1:1000 - 5000
ICC: 1:50 - 100
Species Reactivity
Human
Products Data
Western blot analysis on HeLa cell lysates
using anti-Phospho-Caldesmon (pT753) RabMAb
(cat. # 2394-1), 1:5000 dilution. Cells were
either (A) untreated (B) treated with Calyculin A.
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