Quality Standard:【产品介绍】Carboxypeptidase N is a plasma
metallo-protease that cleaves basic amino acids from the
C terminal of peptides and proteins. CPN1 is important
in the regulation of peptides like kinins
and anaphylatoxins, and it has also been known as
kininase-1 and anaphylatoxin inactivator. It is a
tetramer comprised of two identical regulatory subunits
and two identical catalytic subunits; CPN1 is the
catalytic subunit. Mutations in this protein can
be associated with angioedema or chronic
urticaria resulting from carboxypeptidase N
deficiency (1).
Note: The 48-kDa form is generated by proteolytic
cleavage at the C-terminus. This subunit is cleaved into
a peptide of 27 kDa (2).
Recommended Applications
WB, ICC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:100 - 250
Species Reactivity *
Human
*Species cross-reactivity is based on WB analysis.
Products Data
Western blot analysis on (A) human plasma, (B) HepG2,
and (C) fetal liver lysates using anti-CPN1 RabMAb
(cat. #5322-1).
Specificity
A synthetic peptide corresponding to residues in human
CPN1 was used as an immunogen.