Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
CKB antibody, B-CK antibody, CKBB antibody, Creatine kinase B-type antibody, B-CK antibody, Creatine kinase B chain antibody

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human , Mouse , Rat
【克隆 ID】EPR3927
【用途】WB , IHC , ICC , FC
【抗原分子量】43kDa

Quality Standard：
【产品介绍】The creatine kinases are a family of
enzymes with a highly conserved protein sequence, which
are involved in the maintenance of ATP at sites of
cellular work .Three cytoplasmic
(non-mitochondrial) isoenzymes of creatine kinase (CK)'
are readily identified in human tissue. These isoenzymes
are dimeric molecules with two dissociable
subunits designated as M- (muscle) or B- (brain) type
that can reassociate to form the
electrophoretically distinct MM, BB, or MB isotypes (1).
The protein encoded by creatine kinase B is a
cytoplasmic enzyme involved in energy homeostasis.
The encoded protein reversibly catalyzes the transfer
of phosphate between ATP and various phosphogens such
as creatine phosphate (2). Creatine kinase isoenzymes
are crucial to energy metabolism, particularly in
tissues with high energy requirements, such as
skeletal muscle, heart, brain and spermatozoa (3).

Recommended Applications

WB, IHC, ICC, FC
Applications and Recommended Dilution Factors

WB: 1:10,000 - 50,000
IHC: 1:100 - 500
ICC: 1:100 - 500
FC: 1: 10 - 100
Species Reactivity

Human, Mouse, Rat

Products Data

Western blot analysis on (A) SHSY-5Y (B) Hela (C) Y-79
(D) SW840 cell lysates using anti-Creatine Kinase B
RabMAb (cat. #2830-1).

Immunohistochemical analysis of paraffin-embedded
human glioma tissue using anti-Creatine kinase B
chain RabMAb (cat. #2830-1).

Immunofluorescent staining of HeLa cells using
anti-Creatine kinase B chain RabMAb (cat. #2830-1).

Specificity

A synthetic peptide corresponding to residues near
the C-term of human Creatine Kinase B type was used
as immunogen.

Storage：
Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.