Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
KRT16 antibody, CK16 antibody, FNEPPK antibody, K16 antibody, K1CP antibody, KRT16A antibody, NEPPK antibody, Keratin, type I cytoskeletal 16 antibody, Keratin-16 antibody

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human
【克隆 ID】EP1615Y
【用途】WB , IHC , ICC , FC , IP
【抗原分子量】48kDa

Quality Standard：
【产品介绍】The human type I keratin 16 (CK16)
is constitutively expressed in a number of
complex epithelial tissues, including skin, but is
better known for its induction under conditions
favoring enhanced proliferation or abnormal
differentiation, including wound healing, psoriasis,
and cancer (1). CK16 is expressed in
suprabasal interfollicular epidermis in wound healing
and other pathological conditions associated
with hyperproliferation, such as psoriasis and are
induced when keratinocytes are cultured in vitro.
However, CK16 is also constitutively expressed in
normal suprabasal mucosal and palmoplantar
keratinocytes (2). Pachyonychia congenita (PC) is a group
of inherited ectodermal dysplasias, the
characteristic phenotype being hypertrophic nail
dystrophy. The PC-1 phenotype may be distinguished by
the absence of the epidermal cysts found in PC-2, and it
has been shown to be caused by mutations in either
keratin K16 or its expression partner, the K6a isoform
of K6. Mutations in K16 have also been shown to cause
a milder related phenotype, focal
non-epidermolytic palmoplantar keratoderma (3).

Recommended Applications

WB, IHC, ICC, IP, FC
Applications and Recommended Dilution Factors

WB: 1:2000 - 10,000
IHC: 1:100 - 250
ICC: 1:100 - 250
IP: 1:10 - 100
FC: 1:10 - 100
Species Reactivity *

Human

*Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on HaCat cell lysate
using anti-CK-16 RabMAb (cat. # 2142-1),
dilution 1:10,000.

B. Immunohistochemical analysis of paraffin-embedded
human squamous cervical carcinoma using anti-CK16
RabMAb (cat. #2142-1).

Specificity

A synthetic peptide corresponding to residues near
the N-terminus of human CK-16 was used as an immunogen.

Storage：
Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.