Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
Cytokeratin 4 (KRT4), CK4 antibody, CYK4 antibody, FLJ31692 antibody, K4 antibody, Keratin, type II cytoskeletal 4 antibody, Type-II keratin Kb4 antibody

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human , Rat
【克隆 ID】EPR1598
【用途】WB , IHC , ICC
【抗原分子量】59kDa

Quality Standard：
【产品介绍】Keratins are a family of structurally
related proteins that form the intermediate
filament cytoskeleton in epithelial cells (1). Keratins
are expressed in pairs by epithelial cells in a tissue
and cell specific manner (2). The major
differentiation specific keratins of the buccal
mucosa, nasal, esophageal and anogenital epithelia
are cytokeratin 4 and cytokeratin 13 (2). Cytokeratin 4
and cytokeratin 13 form heterodimers in normal
epithelial cells (3). In humans, cytokeratin 4 is present
in all layers of the epidermis at 10 weeks and
gradually disappears (3). Genetic mutations in cytokeratin
4 cause white sponge nevus (WSN), an autosomal
dominant disorder that affects noncornified
stratified squamous epithelia (4).

Recommended Applications

WB, IHC, ICC
Applications and Recommended Dilution Factors

WB: 1: 1,000 - 10,000
IHC: 1: 100 - 250
ICC: 1: 100 - 250
Species Reactivity *

Human, Rat

*Mouse cross reactivity tested by western blot and IHC
Rat cross reactivity tested by western blot only

Products Data

Western blot analysis on (A) A431 and (B) human uterus
cell lysates using anti-Cytokeratin 4 RabMAb
(cat. # 2843-1).

Immunohistochemical analysis of paraffin-embedded
human tonsil tissue using anti-Cytokeratin 4 RabMAb.
Note positive staining of squamous cells (cat. #2843-1).

Immunofluorescent staining of A431 cells using
anti- Cytokeratin 4 RabMAb (cat. #2843-1).

Specificity

A synthetic peptide corresponding to residues in
human Cytokeratin 4 was used as an immunogen.

Storage：
Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.