Quality Standard:【产品介绍】The human type II keratin 6 (CK6; 56 kDa)
is expressed in a heterogeneous array of epithelial
tissues under normal conditions, but is better known for
its strong induction in stratified epithelia that feature
an enhanced cell proliferation rate or
abnormal differentiation. It has been demonstrated that
CK6 isoform, CK6a, is clearly the dominant K6 isoform
in skin tissue samples and cultured epithelial cell
lines and that the various isoforms are
differentially regulated within and between
epithelial tissue types (1). The murine genome is known
to have two keratin 6 (CK6) genes, mouse CK6, CK6a and
MK6b. These genes display a complex expression pattern
with constitutive expression in the epithelia of
oral mucosa, hair follicles, and nail beds (2).
Pachyonychia congenital (PC) is characterized
by hypertrophic nail dystrophy and associated
ectodermal features. PC-1 subtype is associated
with mutations in keratins 6a or 16, whereas PC-2 subtype
is linked to mutations in keratins 6b or 17 (3).
Recommended Applications
WB, FC
Applications and Recommended Dilution Factors
WB: 1:500
FC: 1:15
Species Reactivity *
Human, Mouse
*Cross reactivity determined by western blot only.
Products Data
A. Western blot analysis on A431 cell lysate of
using anti-CK-6 Rabbit Polyclonal (cat. # 1898-1),
dilution 1:500.
Specificity
A synthetic peptide corresponding to residues on
the N-terminus of human CK-6 was used as an immunogen.