Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
DUX4 antibody, DUX10 antibody, Double homeobox protein 4 antibody, Double homeobox protein 10 antibody, Double homeobox protein 4/10 antibody

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human , Mouse , Rat
【克隆 ID】E55
【用途】WB , FC
【抗原分子量】45kDa

Quality Standard：
【产品介绍】The DUX4 antibody, clone E5-5, was
recently described in the publication
called "Facioscapulohumeral dystrophy:
incomplete suppression of a retrotransposed gene."
(Snider L et al. PLoS Genet. 2010 Oct 28;6(10):e1001181).

DUX4, also known as double homeobox 4, contains
two homeoboxes; the repeat-array and open reading
frame (ORF) is conserved in other mammals. There was
no evidence for transcription from standard cDNA
libraries; however, RT-PCR and in-vitro
expression experiments indicate that DUX4 has been
reported to function as a transcriptional activator
of paired-like homeodomain transcription factor 1.
Mutations in DUX4 cause autosomal
dominant facioscapulohumeral muscular dystrophy (FSHD) (1).

Recommended Applications

WB, FC
Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
FC: 1:10 - 100
Species Reactivity *

Human, Mouse, Rat

*Species cross-reactivity is based on WB analysis.

Products Data

Western blot analysis on (A) human testis, (B) HT-1080,
(C) PC-3, and (D) HeLa lysates using anti-DUX4 RabMAb
(cat. #5158-1).

Flow cytometric analysis of permeabilized HeLa cells
using anti-DUX4 RabMAb (red) (cat.# 5158-1) or a rabbit
IgG (negative) (green).

Specificity

Recombinant proteins, a mixture of two different
protein fragments, of human DUX4 were used as an immunogen.

Storage：
Storage Condition and Buffer

Store at -20 °C. Antibody buffer, 0.01% sodium azide,
50% Glycerol and 0.05% BSA. Stable for 12 months from date
of receipt