Quality Standard:【产品介绍】The eukaryotic translation Elongation Fact
o r 2 (eEF2) is a 95 kDa member of the
G-protein superfamily. Following peptide bond f o r
mation, eEF2 catalyzes translocation of the deacylated
tRNA in the P-site a n d peptidyl tRNA in the A-site
into the E- a n d P- sites, respectively (1). The
activity of eEF2 is regulated by phosph o r ylation (2).
To be active, eEF2 must be dephosph o r ylated, a n d
phosph o r ylation at Thr-56 causes inactivation,
resulting in the termination of mRNA translation (3).
eEF2 is phosph o r ylated by a specific, calcium
a n d calmodulin (Ca/CaM)-dependent eEF2 kinase (4).
WB: 1:1,000
IHC: 1:50-100
ICC: 1:50
A. Western blot analysis on293 cell lysate using
anti-eEF2 RabMAb (cat. # 1681-1), dilution 1:1,000.
B. Immmunohistochemical analysis of paraffin-embedded
human lymphoma using anti-eEF2 RabMAb (cat. # 1681-1).