Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
EGFR antibody, ERBB antibody, ERBB1 antibody, HER1 antibody, PIG61 antibody, mENA antibody, Epidermal growth factor receptor antibody, Proto-oncogene c-ErbB-1 antibody, Receptor tyrosine-protein kinase erbB-1 antibody

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human
【克隆 ID】EP2255Y
【用途】WB , ICC
【抗原分子量】175kDa

Quality Standard：
【产品介绍】Epidermal growth factor receptor (EGFR) is a
175 kDa transmembrane glycoprotein receptor tyrosine
kinase that, activated by epidermal growth factor
(EGF), affects cell growth and differentiation (1).
Binding of EGF or TGF alpha to EGFR activates
tyrosine kinase activity of the receptor (2). The
epidermal growth factor (EGF) receptor is regulated
by EGF-stimulated autophosphorylation and by phorbol
ester-stimulated, protein kinase C (Ca2+/
phospholipid-dependent enzyme) mediated phosphorylation
at identified sites. The EGF receptor contains
additional phosphorylation sites including a
prominent phosphothreonine and several phosphoserines
which account for the majority of phosphate covalently
bound to the receptor in vivo. Analysis of
predicted secondary structure of the EGF receptor
reveals that all of the phosphorylation sites are
located near beta turns (3).

Recommended Applications

WB, ICC
Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
ICC: 1:100 - 250
Species Reactivity *

Human

*Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on A431 cell lysates
using anti-Phospho-EGFR (pT678) RabMAb
(cat. #2344-1), 1:50,000 dilution. Cells were
either (A) untreated (B) treated with EGF

B. Immunofluorescent staining of A431 cells
using anti-Phospho-EGFR RabMAb (cat. # 2344-1).

Specificity

A phospho specific peptide corresponding to
residues surrounding threonine 678 of human EGFR was used
as an immunogen. This antibody detects EGFR
phosphorylated on thr678.

Storage：
Storage Condition and Buffer
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.