Rabbit Monoclonal Antibody

Molecular Formula：

Molecular Weight：

Alias：
F13A1 antibody, F13A antibody, Coagulation factor XIII A chain antibody, Protein-glutamine gamma-glutamyltransferase A chain antibody, Transglutaminase A chain antibody

Product order：

Name	Specifications	Packing	Price	Delivery
Rabbit Monoclonal Antibody		100ul	Inquiry	现货

Character：
【反应特异性】Human
【克隆 ID】EP3372
【用途】WB , IHC , IP
【抗原分子量】80kDa

Quality Standard：
【产品介绍】Factor XIII (plasma transglutaminase,
fibrin stabilizing factor) is a plasma protein that plays
an important role in the final stages of blood
coagulation and fibrinolysis. It circulates in blood as
a tetramer consisting of two "A" and two "B" subunits
(1, 2). The amino acid sequence of the enzymatically
active subunit, Factor XIII A, is unique and does
not exhibit internal homology, but its active center
is similar to that of the thiol proteases. Factor XIII A
is activated by thrombin and calcium ion to
a transglutaminase that catalyzes the cross linking
of fibrin molecules, forming intermolecular
isopeptide bonds, thus stabilizing blood clots (3, 4).
In two diseases that share some histological
resemblance (Lymphocyte-poor graft-versus-host-reaction
and toxic epidermal necrolysis), Factor-XIII
A-positive dendrocytes show some morphological
changes, probably as a response to altered
cytokine environment. Factor-XIII A-positive
dendrocytes thus are reported to possibly play a role in
the regulation of the connective tissue remodeling that
may accompany epidermal destruction (5).

Recommended Applications

WB, IHC, IP
Applications and Recommended Dilution Factors

WB: 1:1,000 - 10,000
IHC: 1:100 - 250
IP: 1:20
Species Reactivity *

Human

*Cross reactivity determined by western blot only.

Products Data

A. Western blot analysis on PBMC cell lysate
using anti-Factor XIII A RabMAb (cat. # 2559-1).

B. Immunohistochemical analysis of paraffin-embedded
human skin tissue using anti-FactirXIII A RabMAb.
Note positive staining of dendrocytes within the
papillary dermis (cat. #2559-1).

Specificity

A synthetic peptide corresponding to residues in
human Factor XIII A was used as an immunogen.

Storage：
Storage Condition and Buffer

Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.