HER2 / ErbB2 Phospho (pY877) (ERBB2)

Molecular Formula：

Molecular Weight：

Alias：
ERBB2 antibody, CD340 antibody, HER-2 antibody, HER-2/neu antibody, HER2 antibody, MLN 19 antibody, NEU antibody, NGL antibody, TKR1 antibody, Receptor tyrosine-protein kinase erbB-2 antibody, Proto-oncogene Neu antibody, Proto-oncogene c-ErbB-2 antibody,

Product order：

Name	Specifications	Packing	Price	Delivery
HER2 / ErbB2 Phospho (pY877) (ERBB2)		100ul	Inquiry	现货

Character：
【产品类型】Rabbit Monoclonal Antibody
【反应特异性】Human
【克隆 ID】EP2324Y
【用途】WB , IHC , ICC
【产品性状】
【阳性对照】
【产品浓度】
【细胞定位】
【抗原分子量】180kDa

Quality Standard：
【产品介绍】HER2 / ErbB2 is one of the four members of
the ErbB receptor family of transmembrane receptor-
like tyrosine kinases (1). The kinase activity of ErbB2
can be activated without ligand if it is overexpressed,
and by association with other ErbB proteins
(2). Overexpression of ErbB2 is detected in almost 40%
of human breast cancers (3). ErbB2 is one of the
major targets for the treatment of breast cancer and
other carcinomas. Autophosphorylation did not
modulate receptor turnover. A Tyr----Phe substitution
of ErbB-2 Tyr-877 homologous to pp60c-src Tyr-416 did
not alter ErbB-2 biological and biochemical properties,
thus excluding the possibility that phosphorylation of
this residue, located in the kinase domain, modulates
ErbB-2 gp185 catalytic function (4).

Recommended Applications
WB, IHC, ICC
Applications and Recommended Dilution Factors
WB: 1:500 - 1000
IHC: 1:250 - 500
ICC: 1:50 - 100
Species Reactivity
Human

Products Data
Western blot analysis on (A,B) HeLa cell lysates or
(C,D) SKBR-3 using anti-Phospho-HER2 (pY877) RabMAb
(Cat. #2521-1) Cells were either (A,C) untreated
(B,D) treated with EGF

Paraffin-embedded human breast ductal infiltrating
carcinoma tissue stained with anti-HER2 Phospho
(pY877) RabMAb. (Cat# 2521-1) Note positive (3+)
membrane staining of tumor cells

Immunofluorescent staining of SKBR3 cells using
anti-HER2 Phospho (pY877) RabMAb. (Cat# 2521-1).

Specificity
A phospho - specific peptide corresponding to
residues surrounding tyrosine 877 of human HER2 was used
as an immunogen. This antibody detects HER2
phosphorylated at tyrosine 877.

Storage：
Store at -20 °C. Buffer: 50 mM Tris-Glycine (pH 7.4),
0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and
0.05% BSA. Stable for 12 months from date of receipt.