Quality Standard：【产品介绍】Interferons (IFNs) plays a crucial role
in antiviral, antiproliferative,
immunomodulatory, antitumor, and antiparasitic activities
by inducing transcription of IFN-stimuated genes
(ISGs) through activation of the Jak-STAT pathway.
In particular, Interferon gamma (IFN γ), a sole member
of the Type II IFN family, is involved in
lymphocyte activation and the regulation of
lymphocyte subsets (proliferation, differentiation) (1).
IFN γ is expressed in activated CD4 Th1 cells, CD8
cells, gamma/delta T cells, and activated NK cells.
IFN γ expression is induced by IL-2, TNF-α and IL-12
and suppressed by IL-4, IL-10, and TGF-β (2). IFN γ
binds to IFN γ receptor (IFNGR), which stimulates
various immuno-responses such as macrophages activation
and enhancement of the type I IFNs anti-viral and
anti-tumor activities. In immunocompetent host cells,
IFN γ protects against tumor development and
directs immunogenic phenotype (3). IFN γ has
been implicated in various human diseases, such as
multiple sclerosis and insulin-dependent diabetes
mellitus (4).
Recommended Applications
WB, ICC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:250 - 500
Species Reactivity *
Human, Mouse, Rat
*Species cross-reactivity is based on WB analysis.
Products Data
Western blot analysis on (A) Molt-4, (B) Jurkat, (C)
lymph node, and (D) human kidney lysates using
anti-IFN gamma RabMAb (cat. #5302-1).
Western blot analysis on 5 ng of IFN gamma
recombinant protein using anti-IFN gamma RabMAb
(cat. #5302-1). Note: molecular weight of
recombinant protein is 17 kDa.
Specificity
A synthetic peptide corresponding to residues in human
IFN gamma was used as an immunogen.
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