Quality Standard：【产品介绍】The NF-kappaB/Rel transcription factors
are present in the cytosol in an inactive state,
complexed with the inhibitory IkappaB-alpha protein
(1-5). In response to many different NF-kappaB-
inducing agents including T-cell mitogens,
proinflammatory cytokines, and viral transactivators,
the inhibitory IkappaB-alpha is rapidly phosphorylated
and degraded (2). Phosphorylation of IkappaB-alpha at
Ser32 and Ser36 has been shown to stimulate conjugation
with ubiquitin and subsequent degradation of
IkappaB-alpha (3). Activation of NF-kappaB can also
be achieved after tyrosine phosphorylation of
IkappaBalpha at tyrosine 42, an event that also
ultimately leads to the dissociation of NF-kappaB
and IkappaBalpha (4). Phosphorylation of IkappaB-alpha
at Ser32 and Tyr42 is critical for activation of
NF-kappaB, which then translocates to the nucleus
and induces transcription of genes that protect
organism (1-5).
Recommended Applications
WB, ICC, IP
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:50 - 100
IP: 1:10 - 100
Species Reactivity
Human
Products Data
Western blot analysis on Hela cell lysates
using anti-Phospho-IkappaB-alpha (pS32) RabMAb
(cat. #2798-1). Cells were either (A) untreated (B)
treated with Calyculin A and TNF-a
Specificity
A phospho specific peptide corresponding to
residues surrounding Serine 32 of human IkappaB-alpha
was used as an immunogen.
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