Quality Standard：【产品介绍】Insulin recept o r substrates (IRS), the maj
o r intracellular substrates of the insulin recept o r
(IR), are adapt o r proteins that transduce signals from
the IR to downstream effect o r s that are imp o r tant
f o r the biological effect of insulin (1-2). After
insulin stimulation, IRS proteins are rapidly phosph
o r ylated on multiple tyrosine residues. Once phosph
o r ylated, IRS proteins bind a n d activate Grb-2,
SHP2 a n d the PI3-K p85 subunit (2-3). Sequences of
IRS-2 a n d IRS-1 reveal a highly conserved amino
terminus containing a pleckstrin-homology domain a n d
a phosphotyrosine-binding domain, a n d a
po o r ly conserved carboxy terminus containing
several tyrosine phosph o r ylation motifs.
IRS-2 is expressed in many cells, including tissues
from IRS-1-/- mice, a n d may be essential f o r
signaling by several recept o r systems such as insulin
a n d cytokine (1).
WB: 1:100,000
IHC: 1:100 250
IP: 1:50
A. Western blot analysis on 293 cell lysate using anti-IRS-
2 RabMAb (cat. #1849-1), dilution 1:100,000
B. Immunohistochemical staining of paraffin-embedded
human kidney using anti-IRS-2 RabMAb (cat. #1849-1).
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