Quality Standard:【产品介绍】JNK3 is a member of the MAP kinase family.
MAP kinases act as an integration point for
multiple biochemical signals, and are involved in a
wide variety of cellular processes such as
proliferation, differentiation, transcription regulation
and development. This protein is a neuronal-specific form
of c-Jun N-terminal kinases (JNKs). Through
its phosphorylation and nuclear localization, this
kinase plays regulatory roles in the signaling
pathways during neuronal apoptosis. Beta-arrestin 2,
a receptor-regulated MAP kinase scaffold protein, is
found to interact with, and stimulate the phosphorylation
of this kinase by MAP kinase kinase 4 (MKK4).
Cyclin-dependent kianse 5 can phosphorylate, and inhibit
the activity of this kinase, which may be important
in preventing neuronal apoptosis (1).
Recommended Applications
WB, ICC, FC
Applications and Recommended Dilution Factors
WB: 1:1,000 - 10,000
ICC: 1:100 - 250
FC: 1:10 - 100
Species Reactivity *
Human, Mouse, Rat
*Species cross-reactivity is based on WB analysis.
Products Data
Western blot analysis on (A) HeLa, (B) 293T,
(C) SH-SY5Y, and (D) human heart lysates using
anti-JNK3 RabMAb (cat. #5474-1).
Immunofluorescent staining of 293 cells using
anti-JNK3 RabMAb (cat. #5474-1).
Specificity
A synthetic peptide corresponding to residues near
the C-terminus of human JNK3 was used as an immunogen.