DNAase
MDL:
MFCD00130918
Molecular Formula:
Molecular Weight:
Alias:
脱氧核糖核酸酶
deoxyribonuclease
Deoxyribonucleate 5′-oligonucleotido-hydrolase
DNase I
Product order:
Name |
Specifications |
Packing |
Price |
Delivery |
DNAase |
≥2,000 Kunitz units/mg protein |
1g |
RMB 1518 |
现货 |
DNAase |
≥2,000 Kunitz units/mg protein |
500mg |
RMB 825 |
现货 |
DNAase |
≥2,000 Kunitz units/mg protein |
100mg |
RMB 231 |
现货 |
DNAase |
≥300 Kunitz units/mg protein |
100mg |
RMB 88 |
Consulting service |
Character:
牛胰腺脱氧核糖核酸酶(又称DNA酶)是一种核酸内切酶,它作用于磷酸二脂键,特别针对毗邻嘧啶核苷的键,从而产生3' 末端带自由羟基和5' 末端带磷酸盐的多聚核苷酸。酶作用的最佳pH值为7.8。DNA酶能被二价金属活化,被鳌合物如EDTA和十二烷基硫酸钠抑制。浓度为5 mM的钙离子可作为稳定剂,以保护DNA酶不被水解酶分解。
该DNA酶是从牛胰腺中提取的,并用层析的方法制备以去除其他水解酶的污染。单位定义: One Kunitz unit will produce
a ΔA260 of 0.001 per min per mL at
pH 5.0 at 25 °C, using DNADNAase,
as substrate. [Mg2+] = 4.2 mM
Quality Standard:
项目 规格
外观Appearance 白色或淡黄色冻干粉末
效价potency ≥2000 Kunitz单位/mg粉末
干燥失重drying loss ≤ 0.3%
灼烧残渣Residue on ignition ≤ 1.0%
Storage:
储存温度 −20°C
用途 用于从蛋白样品中去除DNA